diff --git chrome/browser/chrome_resource_bundle_helper.cc chrome/browser/chrome_resource_bundle_helper.cc
index 0cfc966050b60..8268a8b1f4fcb 100644
--- chrome/browser/chrome_resource_bundle_helper.cc
+++ chrome/browser/chrome_resource_bundle_helper.cc
@@ -82,16 +82,8 @@ std::string InitResourceBundleAndDetermineLocale(PrefService* local_state,
            .empty());
 #endif
 
-  std::string preferred_locale;
-#if BUILDFLAG(IS_MAC)
-  // TODO(markusheintz): Read preference pref::kApplicationLocale in order
-  // to enforce the application locale.
-  // Tests always get en-US.
-  preferred_locale = is_running_tests ? "en-US" : std::string();
-#else
-  preferred_locale =
+  std::string preferred_locale =
       local_state->GetString(language::prefs::kApplicationLocale);
-#endif
 
 #if BUILDFLAG(IS_CHROMEOS_ASH)
   ui::ResourceBundle::SetLottieParsingFunctions(
diff --git components/language/core/browser/locale_util.cc components/language/core/browser/locale_util.cc
index aa43742055b04..e84f21ab963cc 100644
--- components/language/core/browser/locale_util.cc
+++ components/language/core/browser/locale_util.cc
@@ -17,11 +17,11 @@ std::string GetApplicationLocale(PrefService* local_state) {
   // LoadLocaleResources(), which is how the global locale is set.
   // TODO(asvitkine): We should try to refactor things so that the logic is not
   // duplicated in multiple files.
-#if !BUILDFLAG(IS_APPLE)
+
   // The pref isn't always registered in unit tests.
   if (local_state->HasPrefPath(prefs::kApplicationLocale))
     preferred_locale = local_state->GetString(prefs::kApplicationLocale);
-#endif
+
   // Note: The call below is necessary even if |preferred_locale| is empty, as
   // it will get the locale that should be used potentially from other sources,
   // depending on the platform (e.g. the OS locale on Mac).